Key features and details | |
Cat. No. | MABL-3441 |
Name | Anti-Notch 1 mAbs [2E6](MABL-3442) |
Clone No. | 2E6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, Block |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human notch 1. |
Alternative Name | Notch1; hN1; TAN-1; Neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 |
UniProt | P46531 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with a concatemeric protein containing 4 repeats of amino acids 413-488 of human Notch 1 linked by two non-Notch 1 amino acids (AG linker). |
Application Notes | The original human IgG1 version of this antagonist antibody binds notch 1 with an affinity of Kd= 8 nM. This antibody is capable of efficiently blocking the binding of all 4 ligands (Jag1, Jag2, DLL1 and DLL4) to notch 1. This antibody was also capable of specifically inhibiting Notch 1-dependent transcription. In vivo, this antibody was found to show little to no intestinal toxicity in mice. Hu2E6-62 caused decrease in functional angiogenesis by increasing vascular branching and decreasing vascular function. It inhibited angiogenesis induced by the human lung cancer Calu-6 cells. This antibody can be used to inhibit or reduced the proliferation and growth of tumors (WO2012003472). |
Antibody First Published | WO2012003472 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
