Key features and details | |
Cat. No. | MABL-3440 |
Name | Anti-CD56 mAbs [N901](MABL-3441) |
Clone No. | N901 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds CD56, present on large granular lymphocytes including natural killer cells. This antibody does not react with monocytes, granulocytes, B cells, erythrocytes, or platelets. |
Alternative Name | NCAM1; NCAM; NCAM-1; Neural cell adhesion molecule 1 |
UniProt | P13591 |
Immunogen | The original antibody was generated by immunizing mice with human chronic myelogenous leukemia (CML) cells. |
Application Notes | This antibody does not block the natural killer cell activity and can be used to rapidly purify functional NK cells (PMID:6574190). This antibody was able to precipitate a band at approx. 200-250 kdalton protein in a immunoprecipitation assay. This antibody successfully blocked the binding of anti- NKH1A antibody in an indirect immunofluorescence assay (PMID:3884668). This antibody is also recommended to be used in the study of cell surface antigens of the natural cells (PMID: 3875369).Conjugate of DC1 with murine and humanizsed versions of N901 was found to be potent and effective in selective killing of target cells in culture (PMID: 7545085). THis antibody can also be used for the separation of natural killer cells in a flow cytometry (PMID: 15985539). |
Antibody First Published | Griffin et al. Characterization of an antigen expressed by human natural killer cells. J Immunol (1983);130(6):2947-51. PMID:6574190 |
Note on publication | Describes the characterization of the antigen present on human natural killer cells using this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
