Cat. No.
MABL-6193
Type
Shipped
Shipped with Ice Packs
Isotype
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Description
Heparanase 1 (HPA1), Polyclonal Rabbit Anti-Human
Introduction
Heparanase is an endo ß-D-glucuronidase, which degrades heparan sulfate side chains of heparan sulfate proteoglycans (HSPGs) in the extracellular matrix. Heparanase plays an important role in ECM degradation, facilitating the migration and extravasation of tumor cells and inflammatory leukocytes (1,2,3). Upon degradation, heparanase releases growth factors and cytokines that stimulate cell proliferation and chemotaxis (4,5). Heparanase is a heterodimer comprised of a 50 kDa subunit harboring the active site and a 8 kDa subunit. It is produced as a latent 65 kDa precursor and proteolytically processed to its active form (1,6). Heparanase is highly expressed in myeloid leukocytes (i.e. neutrophils) in platelets and in human placenta. Human heparanase was found to be upregulated in various types of primary tumors, correlating in some cases with increased tumor invasiveness and vascularity and with poor prospective survival (7,8).
Formulation
Each vial contains 0.29 mg or 0.57 mg of antibody in 50 or 100 µL, respectively, of 20 mM sodium phosphate; 150 mM NaCl; pH 7.2, containing 0. 01% Thimerosal.
Immunogen
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Applications
Western blot Immunohistochemistry
Stability
Store at 4°C. For extended storage, freeze in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Purification Method
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Purity
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Source
Polyclonal rabbit anti-human HPA1 is a Protein G affinity purified polyclonal antibody raised against the 50 kDa-8 kDa Heparanase heterodimer.
Solubility
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Host
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Physical Appearance
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Storage Procedures
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Specificity
Western blot analysis: The antibody reacts with the 65 kDa precursor as well as the 50 kDa and 8 kDa subunits of human or mouse Heparanase. Immunohistochemistry: The antibody interacts with Heparanase in paraffin sections and blood smears. Recommended dilution range for Western blot analysis: 1:2000. Recommended dilution range for immunohistochemistry: 1:100.
Synonyms
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