Key features and details | |
Cat. No. | MABL-3371 |
Name | Anti-Asp f9 mAbs |
Clone No. | AFD-5C6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Aspergillus fumigatus (Neosartorya fumigata) |
Basic Information | |
Specificity | This antibody recognises Asp f 9. |
Alternative Name | A. fumigatus Allergen; Asp f 9; Aspf9; probable glycosidase crf1; Crh-like protein 1; glucanase; allergen: Asp f 9; allergen Asp f 9 |
UniProt | Q8J0P4 |
Immunogen | 5C6 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion.Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 9 IgE. |
Application Notes | A target of the 5C6 antibody (Asp f 9) was confirmed through IP and mass spectrometry (Wurth et al., 2018). ELISA: this antibody detected Asp f 9 in a commercial Aspergillus extract ALKB in ELISA (Wurth et al., 2018). WB: 5C6 antibody was successful in detecting Asp f 9 in western blot samples from Aspergillus extracts HS and AlgryL (Wurth et al., 2018). |
Antibody First Published | Wurth et al. Human IgE mAbs define variability in commercial Aspergillus extract allergen composition JCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387. PMID:30333320 |
Note on publication | The article describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
