Key features and details | |
Cat. No. | MABL-3372 |
Name | Anti-Il-8 mAbs |
Clone No. | AFD-5.12.14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, inhibition, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises Interleukin-8 (Il-8) which is a neutrophil chemotactic peptide. It is secreted by many different cells which had been activated by inflammatory stimuli. It is specific to Il-8 and does not bind to other chemokins, such as C5a, platelet factor 4 or β-TG. |
Alternative Name | Interleukin-8; Il8; II-8; Il 8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor |
UniProt | P10145 |
Immunogen | This antibody was raised by immunising Balc/c mice against conjugated Il-8. |
Application Notes | This antibody is an Il-8 antagonist and prevents binding to neutrophils, which might be used in research aiming at treating asthma (before or after its onset) (US5874080A). Furthermore, this antibody inhibits both Il-8-dependent neutrophil chemotaxis and neutrophil elastase release (US5874080A). |
Antibody First Published | Hebert et al. Anti-IL-8 monoclonal antibodies for treatment of asthma Hebert, C.A., Kabakoff, R.C. and Moore, M.W., Genentech Inc, 1999. Anti-IL-8 monoclonal antibodies for treatment of asthma. U.S. Patent 5,874,080. PMID: |
Note on publication | The article describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
