Key features and details | |
Cat. No. | MABL-3373 |
Name | Anti-Gliadin mAbs |
Clone No. | AFD-VHH26 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Wheat |
Basic Information | |
Specificity | This antibody specifically recognizes and binds and epitope unique to wheat alpha gliadins. |
Alternative Name | Alpha-gliadin; α-gliadin; Celiac Allergen; Coeliac Allergen |
UniProt | Q306G0 |
Immunogen | The original antibody was prepared by immunization of Llama glama with whole gliadin. |
Application Notes | This antibody specifically binds an epitope unique for wheat alpha gliadins which is not present in gamma or omega gliadins. It does not cross reat with rye or barley gliadins. VHH26 can detect gliadins in denaturing conditions and it retains its binding capacity in presence of 15% ethanol, 0.5% 2-mercaptoethanol, and 0.5 M guanidinium chloride. Capture ELISA using VHH26 was able to detect gliadins in samples shown as negatives by conventional ELISA. This antibody can be used for detection of gluten in commercial food samples using ELISA (limit of detection 0.24 ppm) and by competitive ELISA using conventional antibodies (limit of detection: 1 ppm) (PMID: 20039674). |
Antibody First Published | Doña et al. Single domain antibodies are specially suited for quantitative determination of gliadins under denaturing conditions. J Agric Food Chem (2010); 58(2): 918-26.PMID:20039674 |
Note on publication | This patent describes the generation and characterisation of the 5.12.14 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
