Key features and details | |
Cat. No. | MABL-3374 |
Name | Anti-Gliadin mAbs |
Clone No. | AFD-PEE14C7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | barley, rye, spelt, triticale, Wheat |
Basic Information | |
Specificity | This antibody specifically recognizes and binds deaminated gliadens and glutens. It recognizes the epitope sequence 'QPEEPFPE' and does not show any cross reactivity towards non-deamidated gliadin and gluten proteins. |
Alternative Name | Deaminated gliadin; Celiac Allergen; Coeliac Allergen; Alpha-gliadin; α- gliadin; β gliadin; Beta-gliadin; γ gliadin; Gamma-gliadin; Omega-2 gliadin; Omega-5 gliadin; Omega gliadin; ω gliadin |
UniProt | |
Immunogen | The original antibody was generated by immunizing mice with a solution of peptide 'QPEEPFPE' conjugated to KLH and of a solution of adjuvant . |
Application Notes | This antibody is recommended for detection of deaminated gliadins and glutenins from wheat, rye, barley, spelt and triticale in commercial food samples (Tranquet et al., 2012). This antibody can also be used in the detection of certain allergies to deaminated glutens (PMID: 22737987). |
Antibody First Published | Tranquet et al. Selection of a monoclonal antibody for detection of gliadins and glutenins: A step towards reliable gluten quantification. Journal of Cereal Science (2012); Volume 56; Issue 3; Pages 760-763. PMID: |
Note on publication | Describes the generation and specific use of this antibody in the quantitive determination of gliadins. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
