Key features and details | |
Cat. No. | MABL-3376 |
Name | Anti-Gliadin mAbs |
Clone No. | AFD-1E01 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | AlphaLISA, ELISA, FC |
Species Reactivity | barley, rye, spelt, triticale, Wheat |
Basic Information | |
Specificity | This antibody can recognize and bind the 'QPEQPFP' peptide of gliadins. This antibody also reacts with deaminated 33mer peptide of α-gliadin 'LQLQPFPQPELPYPQPELPYPQPELPYPQPQPF '. |
Alternative Name | gluten; Celiac Allergen; Coeliac Allergen |
UniProt | |
Immunogen | The original antibody was isolated from the IgA+ plasma cells of celiac patients gut lesions that are reactive with deaminated gluten proteins. |
Application Notes | The binding specificity of IgA+ plasma cells in the single cell suspension was determined by flow cytometry. The relative affinity of the antibody to a panel of gliadin was investigated using ELISA and AlphaLISA (PMID: 27146306, WO2015164615). This antibody recognizes a sequence that intimately associates with deamidation of the gluten peptide. (PMID:28878138). |
Antibody First Published | Dorum et al. Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes. Sci Rep. (2016); 6: 25565.PMID:27146306 |
Note on publication | Describes the generation and epitope characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
