Key features and details | |
Cat. No. | MABL-3377 |
Name | Anti-Pancreatic alpha-amylase mAbs |
Clone No. | AFD-AMD9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Pig (Sus scrofa) |
Basic Information | |
Specificity | This antibody binds the active site crevice of the porcine pancreatic alpha-amylase and is a strong amylase inhibitor. |
Alternative Name | PA; AMY2; 1,4-alpha-D-glucan glucanohydrolase |
UniProt | P00690 |
Immunogen | The original antibody was generated by immunizing dromedary with the enzyme and isolating specific binders from the VHH cloned from peripheral blood lymphocytes. |
Application Notes | This antibody can bind porcine pancreatic alpha-amylase in an ELISA and inibits the enzyme with an IC50 = 10nM (PMID: 9649422). This antibody interacts with the amino acid residues in the alpha amylase catalytic site and has a strong inhibitary action on the catalytic activity of the enzyme. The inhibitory interaction of AMD9 involved mainly its complementarity-determining region (CDR) 2 loop, whereas the CDR3 loop was small and certainly did not protrude (PMID: 11960990). |
Antibody First Published | Lauwereys et al. Potent enzyme inhibitors derived from dromedary heavy- chain antibodies. The EMBO Journal (1998), Vol.17 No.13, pp.3512–3520. PMID:9649422 |
Note on publication | Describes the generation and epitope characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
