Cat. No.
MAK-0137
Product Name
Dog AGP
Storage
2-8°C
Species
Dog
Introduction
AGP is an acute phase protein that is elevated in dog serum due to injury, infection or disease. Studies have demonstrated that serum AGP levels may increase nine-fold or more in dogs during the acute phase response.
Principle Of The Assay
The assay uses affinity purified dog AGP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated dog AGP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in AGP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and
TMB is added and incubated for 20 minutes. If AGP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of AGP is proportional to absorbance and is derived from a standard curve.
