Cat. No.
MAK-0140
Product Name
Cat AGP
Storage
4°C
Species
Cat
Introduction
AGP is an acute phase protein that is elevated in cat serum due to injury, infection, and disease. Kajikawa, et al. reported a 19-fold increase of AGP in serum from hospitalized cats and a 5.7-fold increase after injection of normal cats with lipopolysaccharide. Of the positive acute phase proteins investigated (SAA, AGP, haptoglobin and CRP), they reported that AGP was the most responsive. More recently, Paltrinieri et al., reported AGP to be a powerful discriminating biomarker for diagnosis of feline infectious peritonitis.
Principle Of The Assay
The assay uses affinity purified cat AGP antibodies for solid phase
(microtiter wells) immobilization and horseradish peroxidase (HRP)
conjugated cat AGP antibodies for detection. Standards and diluted
samples are incubated in the microtiter wells for 45 minutes. The
wells are subsequently washed. HRP conjugate is added and
incubated for 45 minutes. This results in AGP molecules being
sandwiched between the immobilization and detection antibodies.
The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If AGP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of AGP is proportional to absorbance and is derived from a standard curve.
