Cat. No.
MAK-0155
Product Name
Turtle MRP-126
Storage
4°C
Species
Turtle
Introduction
MRP-126 (S100-A12) belongs to the calgranulin protein family. It is a homodimer that calcium dependently sequesters zinc, thereby inhibiting bacterial growth. Proteomic studies indicate that levels increase in plasma of health-compromised turtles.
Principle Of The Assay
The assay uses a polyclonal antibody for green turtle MRP-126. Unconjugated antibody is coated on wells of a microtiter plate and used for capture. Horseradish peroxidase (HRP) conjugated antibody is used for detection. Standards and diluted samples (100 ul) are incubated in the antibody coated wells for 45-minutes. After washing the wells, HRP-conjugate (100 ul) is added and incubated for 45 minutes. If MRP-126 molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If MRP-126 is present, a blue color develops. Color development is stopped by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of MRP-126 is proportional to absorbance and is derived from a standard curve.
