Cat. No.
MAK-0161
Product Name
Rat Clusterin
Storage
4°C
Species
Rat
Introduction
Clusterin, also referred to as apolipoprotein J, sulfated glycoprotein, glycoprotein III, and testosterone-repressed prostate message-2, is a glycoprotein of 70-80 kDa.Clusterin is expressed in many tissues and is found in serum, seminal fluid, and urine. It has been identified as a potential biomarker of various forms of renal injury and prostate disease.
Principle Of The Assay
The assay uses affinity purified rat clusterin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rat clusterin antibodies for detection. Standards and diluted samples are added to appropriate wells of the plate. HRP conjugated antibody is then added to the wells and the plate is incubated on a plate shaker for one hour at room temperature. This results in clusterin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If clusterin is present a blue color develops. Color development is stopped by the addition of Stop Solution, changing
the color to yellow. Absorbance is measured at 450 nm. The concentration of clusterin is proportional to absorbance and is derived from a standard curve.
