Cat. No.
MAK-0166
Product Name
Rat Haptoglobin
Storage
2-8°C
Species
Rat
Introduction
Haptoglobin is an acute phase protein that is elevated in rat serum due to injury, infection or disease. Measurement of haptoglobin provides a useful marker of inflammation/disease in rats; levels increase up to ten-fold.
Principle Of The Assay
The assay uses affinity purified rat haptoglobin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rat haptoglobin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 30 minutes. This results in haptoglobin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound
HRP-conjugate and TMB is added and incubated for 20 minutes. If haptoglobin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow,
and absorbance is measured at 450 nm. The concentration of haptoglobin is proportional to absorbance and is derived from a standard curve.
