Cat. No.
MAK-0172
Product Name
Horse Haptoglobin
Storage
2-8°C
Species
Horse
Introduction
Haptoglobin is an acute phase protein that is elevated up to ninefold in horse serum as a result of inflammation and infection.Measurement of haptoglobin therefore provides a convenient marker of inflammation and disease in horses.
Principle Of The Assay
The horse haptoglobin test kit is based on a solid phase enzymelinked immunosorbent assay (ELISA). The assay uses affinity purified anti-horse haptoglobin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-horse haptoglobin antibodies for detection. Serum or plasma is denatured and subsequently diluted. The diluted sample is incubated in the microtiter wells for 45 minutes. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. This results in haptoglobin molecules being sandwiched between the immobilization and detection
antibodies. The wells are then washed to remove unbound HRPlabeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of haptoglobin is proportional to the optical density of the test sample.
