Cat. No.
MAK-0195
Product Name
Monkey SAA
Storage
4°C
Species
Monkey
Introduction
SAA is an acute phase serum protein that can be elevated up to 400fold-fold in monkeys.1,2 As is the case in humans, measurement of SAA provides an excellent biomarker of inflammation and disease.
Principle Of The Assay
The assay uses two different peptide-specific monkey SAA antibodies; one for solid phase immobilization and the other, conjugated to horseradish peroxidase (HRP), for detection. Serum samples are first denatured by heating for 1 hour at 60°C. The denaturing step dissociates SAA from interfering factors. Subsequently, the denatured samples are diluted. Standards and diluted samples are incubated, in the microtiter wells, together with HRP conjugate for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color develops. Color development is stopped by addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm.
The concentration of SAA is proportional to absorbance and is derived from a standard curve.
