Cat. No.
MAK-0196
Product Name
Dog SAA
Storage
4°C
Species
Dog
Introduction
SAA, a protein of approximately 12 kDa, is a positive acute phase reactant that circulates in blood mostly associated with high density lipoprotein. In dogs, basal serum levels are <2 µg/ml but levels can reach 500 µg/ml during infection, disease and cancer.
Principle Of The Assay
The assay uses two different peptide-specific dog SAA antibodies; one for solid phase immobilization and the other, conjugated to horseradish peroxidase (HRP), for detection. Standards and diluted samples are incubated in the microtiter wells, together with HRP conjugate for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color
develops. Color development is stopped by addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is
derived from a standard curve.
