Cat. No.
MAK-0194
Product Name
Mouse SAA
Storage
4°C
Species
Mouse
Introduction
SAA is an acute phase serum protein that is elevated in mice approximately 50-fold following lipopolysaccharide injection. Two major forms of SAA are induced during the acute phase response, SAA1 and SAA2. Studies have shown that the two forms are similarly increased in response to different inflammatory stimuli.
Principle Of The Assay
The assay uses affinity purified peptide-specific polyclonal antimouse SAA2 antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated polyclonal peptide-specific anti-mouse SAA1/2 antibodies for detection. Standards and diluted samples are incubated, together
with HRP conjugate, in the microtiter wells for one hour. This results in SAA molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound
HRP-conjugate. TMB is added and incubated for 20 minutes. If SAA is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived from a standard curve.
