Cat. No.
MAK-0198
Product Name
Guinea Pig SAA
Storage
4°C
Species
Guinea Pig
Introduction
Serum amyloid A (SAA), a protein of approximately 12 kDa, is a positive acute phase reactant that is produced in liver and circulates in blood. Serum levels are increased during inflammation and infection.
Principle Of The Assay
The assay uses two peptide-specific polyclonal antibodies generated against guinea pig SAA. Standards and samples (100 μl) are incubated in anti-SAA coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 μl) is added and incubated for 45 minutes. If SAA molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRPconjugate. TMB is added and incubated for 20 minutes. If SAA is present, a blue color develops. Color development is stopped after 20-minutes by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of SAA is proportional to absorbance and is derived from a standard curve.
