Cat. No.
MAK-0212
Product Name
Rat Transferrin
Storage
2-8°C
Species
Rat
Introduction
Transferrin is a glycosylated serum protein with a molecular weight of 80,000 that serves as an iron carrier in blood. In rats it is a negative acute phase reactant, the serum levels of which decrease by 25 - 40% within 18-24 hours of induction of the acute phase response.
Principle Of The Assay
The assay uses affinity purified anti-transferrin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-transferrin antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in transferrin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If transferrin is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of transferrin is proportional to absorbance and is
derived from a standard curve.
