Name
DCTD Human
Cat. No.
MAG-2986
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
DCTD Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids (1-178 a.a.) and having a molecular mass of 22.1 kDa. The DCTD is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
Introduction
DCTD is an allosteric enzyme that exists as a homohexamer and is part of the cytidine and deoxycytidylate deaminase protein family. DTCD uses zinc as a cofactor to catalyze the deamination of dCMP to dUMP, thus making the nucleotide substrate (dUMP) that is used by thymidylate synthase.
Purification Method
/
Physical Appearance
Sterile filtered colorless solution.
Formulation
The DCTD solution (0.5mg/ml) contains 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA & 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
/
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Deoxycytidylate deaminase, EC 3.5.4.12, dCMP Deaminase, DCTD, MGC111062.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ.
Background
/
