Name
Pfu-sso7d DNA Polymerase
Cat. No.
MAG-3100
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
Pfu-sso7d DNA Polymerase is derived from E. coli, having a molecular mass of 100 kDa. The Pfu-sso7d DNA Polymerase possesses the following activities: 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity. It generates blunt ends in the amplification products.
Introduction
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Purification Method
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Physical Appearance
Sterile liquid formulation.
Formulation
20mM Tris-HCl (pH 8.0), 50% glycerol, 0.1mM EDTA, 100mM KCl, 1mM DTT, 0.1% Tween20 and 0.1% NP-40.
Stability
Store Pfu-sso7d DNA Polymerase at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95 % as determined by SDS-PAGE analyses
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
DNA polymerase, EC 2.7.7.7, Pfu polymerase, Pfu-DNA Polymerase.
Amino acid sequence
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Background
The Pfu-sso7d DNA Polymerase preforms very well for all major PCR applications. The Pfu-sso7d DNA Polymerase generates long amplicons with accuracy and speed. The high fidelity makes the Pfu-sso7d DNA Polymerase a great choice for cloning. The error rate of Pfu-sso7d DNA Polymerase is approximately 50 fold lower than that of the Thermus aquaticus DNA polymerase. It generates blunt ends in the amplification products.
