Name
GZMB Mouse
Cat. No.
MAG-3201
Tag/Conjugates
His
Source
Sf9,
Baculovirus cells.
Shipped
Shipped with Ice Packs
Description
GZMB Mouse produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 235 amino acids (19-247 aa) and having a molecular mass of 26.3kDa. GZMB is fused to a 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.
Introduction
GZMB or Granzyme-B is a protein that mainly exist in granules of NK cells and T cells (cytotoxic), it is a serine protease. The protein is secreted by of NK cells and T cells together with the protein that creates pores (perforin) in order to lead to apoptosis in the target cell.
Purification Method
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Physical Appearance
Sterile Filtered colorless solution.
Formulation
The GZMB solution (0.25mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4).
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
Defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37C ˚ . Specific activity is > 9,000 pmol/min/ug.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Granzyme B, C11, CTLA-1, Cathepsin G-like 1, CTSGL1, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Fragmentin-2, Granzyme-2, Human lymphocyte protein, HLP, SECT, T-cell serine protease 1-3E, CGL1, CSPB, CTLA1, GRB, GZMB, CCPI, CGL-1, CSP-B.
Amino acid sequence
GEIIGGHEVK PHSRPYMALL SIKDQQPEAI CGGFLIREDF VLTAAHCEGS IINVTLGAHN IKEQEKTQQV IPMVKCIPHP DYNPKTFSND IMLLKLKSKA KRTRAVRPLN LPRRNVNVKP GDVCYVAGWG RMAPMGKYSN TLQEVELTVQ KDRECESYFK NRYNKTNQIC AGDPKTKRAS FRGDSGGPLV CKKVAAGIVS YGYKDGSPPR AFTKVSSFLS WIKKTMKSS H HHHHH
Background
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