Name
TOP1 Bovine
Cat. No.
MAG-3292
Tag/Conjugates
His
Source
Bovine tissues.
Shipped
Shipped with Ice Packs
Description
Bovine DNA Topoisomerase-I shows multiple bands between 76-109 KDa and is purified from bovine tissues by proprietary chromatographic techniques.
Introduction
TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.
Purification Method
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Physical Appearance
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Formulation
TOP1 is supplied in 20mM HEPES buffer pH-7.5, 400mM NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.
Amino acid sequence
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Background
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