Name
T4 DNA
Cat. No.
MAG-3329
Tag/Conjugates
His
Source
E.Coli, cloned gene-30, bacteriophage T4.
Shipped
Shipped with Ice Packs
Description
T4 DNA Ligase 55.3kDa protein catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Introduction
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Purification Method
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Physical Appearance
400U/ul solution.
Formulation
50% glycerol, 2 0 mM Tris-HCl (pH-7.5), 50mM KCl, 1mM DTT and 0. 1 mM EDTA.
Stability
Store T4 DNA Ligase at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
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Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
Amino acid sequence
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Background
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