Name
DNase Human
Cat. No.
MAG-3431
Tag/Conjugates
His
Source
Chinese Hamster Ovary Cells.
Shipped
Shipped with Ice Packs
Description
Deoxyribonuclease I Human Recombinant produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C 1321 H 1999 N 339 O 396 S 9 . DNase is purified by proprietary chromatographic techniques.
Introduction
Deoxyribonuclease I Human Recombinant (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2 . Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.
Purification Method
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Physical Appearance
Sterile liquid colorless solution at a concentration of 1mg/ml.
Formulation
Each mg contains 150 µg calcium chloride dehydrate and 8.77 mg NaCl.
Stability
2 years when stored at 4°C, three weeks at 15°C, pH-6.3.
Purity
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Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
EC 3.1.21.1, Deoxyribonuclease I, DNase I, DNL1, DRNI, FLJ38093, DNASE1.
Amino acid sequence
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Background
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