Name
PTPN11 Human, Active
Cat. No.
MAG-3483
Tag/Conjugates
His
Source
Sf9, Insect cells.
Shipped
Shipped with Ice Packs
Description
PTPN11 Human produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 602 amino acids ( 1-593 a.a.) and having a molecular mass of 69.1 kDa. PTPN11 is expressed with a 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction
Protein Tyrosine Phosphatase Non Receptor Type-11 or PTPN11 has 2 Src homology 2 domains and part of the tyrosine phosphatase group of proteins. PTPN11 is responsible for the catalyzation of tyrosine residues dephosphorylation in proteins and takes part in the stimulation and activation of Erk/MAP kinase transduction via signals from tyrosine kinase. Noonan syndrome and acute myeloid leukemia can be caused from mutations in PTPN11.
Purification Method
/
Physical Appearance
Sterile Filtered clear solution.
Formulation
PTPN11 protein solution (1mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
Specific activity is grather than 400 unit/mg and is defined as the amount of enzyme that hydrolyze 1.0 nmole of pnitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
PTPN11, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D, PTP-1D, Proteintyrosine phosphatase 2C, PTP-2C, SH-PTP2, SH-PTP3, BPTP3, CFC, JMML, METCDS, NS1, SHP-2, shp-2, PTP2C, SHPTP2.
Amino acid sequence
ADP MTSRRWF HPNITGVEAE NLLLTRGVDG SFLARPSKSN PGDFTLSVRR NGAVTHIKIQ NTGDYYDLYG GEKFATLAEL VQYYMEHHGQ LKEKNGDVIE LKYPLNCADP TSERWFHGHL SGKEAEKLLT EKGKHGSFLV RESQSHPGDF VLSVRTGDDK GESNDGKSKV THVMIRCQEL KYDVGGGERF DSLTDLVEHY KKNPMVETLG TVLQLKQPLN TTRINAAEIE SRVRELSKLA ETTDKVKQGF WEEFETLQQQ ECKLLYSRKE GQRQENKNKN RYKNILPFDH TRVVLHDGDP NEPVSDYINA NIIMPEFETK CNNSKPKKSY IATQGCLQNT VNDFWRMVFQ ENSRVIVMTT KEVERGKSKC VKYWPDEYAL KEYGVMRVRN VKESAAHDYT LRELKLSKVG QGNTERTVWQ YHFRTWPDHG VPSDPGGVLD FLEEVHHKQE SIMDAGPVVV HCSAGIGRTG TFIVIDILID IIREKGVDCD IDVPKTIQMV RSQRSGMVQT EAQYRFIYMA VQHYIETLQR RIEEEQKSKR KGHEYTNIKY SLADQTSGDQ SPLPPCTPTP PCAEMREDSA RVYENVGLMQ QQKSFR HHHH HH
Background
/
