Name
HPD Mouse
Cat. No.
MAG-3492
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
HPD Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 416 amino acids (1-393 a.a) and having a molecular mass of 47.4kDa. HPD is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
HPGD is the essential enzyme of prostaglandin degradation. 15-PGDH protein strongly decreases the biologic activity of these molecules by catalyzing the oxidation of the 15-hydroxyl group of prostaglandins to a keto group. GDH1 is involved in numerous physiologic and cellular processes, for instance inflammation.
Purification Method
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Physical Appearance
Sterile Filtered clear solution.
Formulation
HPD protein solution (0.5mg/ml) contains 10% glycerol & 20mM Tris-HCl (pH 8.0).
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioways products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, F Alloantigen, F protein.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MTTYNNK GPKPERGRFL HFHSVTFWVG NAKQAASFYC NKMGFEPLAY RGLETGSREV VSHVIKQGKI VFVLCSALNP WNKEMGDHLV KHGDGVKDIA FEVEDCDHIV QKARERGAKI VREPWVEQDK FGKVKFAVLQ TYGDTTHTLV EKINYTGRFL PGFEAPTYKD TLLPKLPRCN LEIIDHIVGN QPDQEMQSAS EWYLKNLQFH RFWSVDDTQV HTEYSSLRSI VVTNYEESIK MPINEPAPGR KKSQIQEYVD YNGGAGVQHI ALKTEDIITA IRHLRERGTE FLAAPSSYYK LLRENLKSAK IQVKESMDVL EELHILVDYD EKGYLLQIFT KPMQDRPTLF LEVIQRHNHQ GFGAGNFNSL FKAFEEEQAL RGNLTDLEPN GVRSGM.
Background
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