Name
Carboxypeptidase B Rat
Cat. No.
MAG-3512
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
At Room Temperature
Description
Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 31kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA.
Introduction
Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kDa, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline.
Purification Method
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Physical Appearance
Sterile Filtered lyophilized powder.
Formulation
The protein was lyophilized with 100mM NaCl, mannitol and 20mM Tris pH-7.5.
Stability
Store the lyophilized Carboxypeptidase-B at 4°C. Upon reconstitute the protein should be stored at 4°C for 2 weeks and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Biological Activity
170 units/mg protein.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Carboxypeptidase B, Cpb1, Cpb.
Amino acid sequence
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Background
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