Name
PPP3R1 Human
Cat. No.
MAG-3626
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
PPP3R1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 190 amino acids (1-170.a.a) and having a molecular mass of 21.5kDa. PPP3R1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. Calcineurin is composed of two subunits; calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells.
Purification Method
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Physical Appearance
Sterile filtered colorless solution.
Formulation
PPP3R1 Human solution (1mg/1ml) containing 20mM Trsi HCL pH-8, 0.1M NaCl, 2mM DTT and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Protein phosphatase 3 regulatory subunit B alpha, CNB1, CALNB1, Protein phosphatase 3 (formerly 2B) regulatory subunit B (19kD) alpha isoform (calcineurin B type I), Calcineurin B type I (19kDa).
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGNEASYPLE MCSHFDADEI KRLGKRFKKL DLDNSGSLSV EEFMSLPELQ QNPLVQRVID IFDTDGNGEV DFKEFIEGVS QFSVKGDKEQ KLRFAFRIYD MDKDGYISNG ELFQVLKMMV GNNLKDTQLQ QIVDKTIINA DKDGDGRISF EEFCAVVGGL DIHKKMVVDV
Background
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