Name
T7 RNAP
Cat. No.
MAG-3663
Tag/Conjugates
His
Source
T7 Bacteriophage RNA Polymerase gene
Shipped
Shipped with Ice Packs
Description
T7 RNA polymerase Recombinant protein is produced by bacteriophage T7 DNA which is expressed in recombinant E. coli bacterial system T7 RNA Polymerase is a DNA-dependent 5'→ 3' RNA polymerase which specifically recognizes T7 promoter sequences.
Introduction
T7 RNA polymerase active enzyme synthesizes RNA at an increasing degree of that of E. coli RNA polymerase and it terminates transcription often. T7 RNA polymerase is very selective for initiation at its own promoter sequences and is resistant to antibiotics that inhibit E. coli RNA polymerase. In-vitro transcription of mRNA is achieved via bacteriophage T7 RNA polymerase using its ability to produce full-length RNA transcripts with high reliability, thoughT7 RNAP can manufacture as well immunostimulatory by products for example dsRNA which affect protein expression.
Purification Method
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Physical Appearance
Sterile Filtered clear solution.
Formulation
Transcription Buffer 40mM Tris-HCl (25°C, pH-8), 20mM MgCl 2 , 2.5mM TCEP & 2mM spermidine.
Stability
Two years when stored at -20°C, 2 weeks at 4°C. DO NOT STORE AT -70C.
Purity
Greater than 95% as visualized by SDS-PAGE
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
T7 RNAP.
Amino acid sequence
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Background
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