Name
PSMB7 Human
Cat. No.
MAG-3701
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
PSMB7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 255 amino acids (44-277) and having a molecular mass of 27.6kDa (Molecular size on SDS-PAGE will appear higher). PSMB7 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Proteasome subunit beta type-7 (PSMB7) belongs to the proteasome B-type family. PSMB7 is a multicatalytic proteinase complex with an extremely ordered ring-shaped 20S core structure. This core structure is comprised of 4 rings of 28 non-identical subunits; 2 rings are comprised of 7 alpha subunits and 2 rings are comprised of 7 beta subunits. Proteasomes are scattered all over eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent manner in a non-lysosomal pathway. The processing of class I MHC peptides is a vital function of the immunoproteasome (which is a modified proteasome).
Purification Method
/
Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PSMB7 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Biological Activity
/
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, PSMB7, Z.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH M TTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTA ADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPY TVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS.
Background
/
