Name
PSMD9 Human
Cat. No.
MAG-3705
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
PSMD9 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (1-223) and having a molecular mass of 27.1kDa. PSMD9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
PSMD9 is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. PSMD9 acts as a chaperone through the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex compilation, PSMD9 is part of an intermediate PSMD9:PSMC6:PSMC3 module, also called modulator trimer complex. PSMD9 is released during the further base assembly process.
Purification Method
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Physical Appearance
Sterile Filtered colorless solution.
Formulation
The PSMD9 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
p27, Rpn4, 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27, PSMD9.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR.
Background
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