Name
NEK7 Human
Cat. No.
MAG-3757
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
NEK7 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 326 amino acids (1-302 a.a) and having a molecular mass of 37kDa.NEK7 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
NEK7, protein kinase, has a vital role in mitotic cell cycle progression. It is essential for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. NEK7 is greatly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen.
Purification Method
/
Physical Appearance
Sterile Filtered clear solution.
Formulation
The NEK7 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) , 0.15M NaCl and 20% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Biological Activity
/
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
NIMA (never in mitosis gene a)-related kinase 7, Serine/threonine-protein kinase Nek7.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSH MDEQSQ GMQGPPVPQF QPQKALRPDM GYNTLANFRI EKKIGRGQFS EVYRAACLLD GVPVALKKVQ IFDLMDAKAR ADCIKEIDLL KQLNHPNVIK YYASFIEDNE LNIVLELADA GDLSRMIKHF KKQKRLIPER TVWKYFVQLC SALEHMHSRR VMHRDIKPAN VFITATGVVK LGDLGLGRFF SSKTTAAHSL VGTPYYMSPE RIHENGYNFK SDIWSLGCLL YEMAALQSPF YGDKMNLYSL CKKIEQCDYP PLPSDHYSEE LRQLVNMCIN PDPEKRPDVT YVYDVAKRMH ACTASS.
Background
/
