Name
Staphylokinase
Cat. No.
MAG-3795
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
At Room Temperature
Description
Staphylokinase Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 136 amino acids and having a molecular weight of 16kDa. The Staphylokinase is purified by proprietary chromatographic techniques.
Introduction
Staphylokinase (SAK) is a 136-amino acidenzymefrom Staphylococcus aureus. It is positively regulated by the "agr" gene regulator. It activates plasminogen, which in turn can degrade various host proteins during infection.
Purification Method
/
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4.
Stability
Lyophilized SAK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SAK should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Biological Activity
The biological activity measured by the ability of fibrin lysis in agarose plate was found to be 50,000IU/mg.
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Staphylokinase, SakSTAR, Neutral proteinase, Protease III, SAK.
Amino acid sequence
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Background
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