Name
TIE1 Fc Mouse
Cat. No.
MAG-3959
Tag/Conjugates
His
Source
CHO Cells.
Shipped
At Room Temperature
Description
Soluble TIE-1 Mouse Recombinant fused with the Fc part of human IgG 1 produced in CHO cells is a glycosylated disulfide-linked homodimeric polypeptide chain containing amino acids 23-749 and having a total molecular mass of 260kDa.
Introduction
TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Whereas two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], so far no ligand was found for TIE-1.
Purification Method
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Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
TIE-1 Fc Chimera was lyophilized from a concentrated (1mg/ml) sterile solution containing 1x PBS.
Stability
Lyophilized sTIE-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIE-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1, JTK14, TIE, TIE1.
Amino acid sequence
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Background
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