Name
ACAA2 Human
Cat. No.
MAG-3989
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
ACAA2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 404 amino acids (17-397) and having a molecular mass of 42.6kDa. ACAA2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Acetyl-COA Acyltransferase 2, (ACAA2) is a member of the thiolase family. ACAA2 catalyzes the final step of the mitochondrial fatty acid beta-oxidation spiral. Not like most mitochondrial matrix proteins, ACAA2 contains a non-cleavable amino-terminal targeting signal.
Purification Method
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Physical Appearance
Sterile Filtered clear solution.
Formulation
The ACAA2 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
DSAEC, 3-ketoacyl-CoA thiolase, mitochondrial, Acetyl-CoA acyltransferase, Beta-ketothiolase.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS FGAYGGL LKDFTATDLS EFAAKAALSA GKVSPETVDS VIMGNVLQSS SDAIYLARHV GLRVGIPKET PALTINRLCG SGFQSIVNGC QEICVKEAEV VLCGGTESMS QAPYCVRNVR FGTKLGSDIK LEDSLWVSLT DQHVQLPMAM TAENLAVKHK ISREECDKYA LQSQQRWKAA NDAGYFNDEM APIEVKTKKG KQTMQVDEHA RPQTTLEQLQ KLPPVFKKDG TVTAGNASGV ADGAGAVIIA SEDAVKKHNF TPLARIVGYF VSGCDPSIMG IGPVPAISGA LKKAGLSLKD MDLVEVNEAF APQYLAVERS LDLDISKTNV NGGAIALGHP LGGSGSRITA HLVHELRRRG GKYAVGSACI GGGQGIAVII QSTA.
Background
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