Name
LCMT1 Human
Cat. No.
MAG-4070
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
LCMT1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 358 amino acids (1-334) and having a molecular mass of 41kDa. LCMT1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
LCMT1 is a member of the LCMT family, methyltransferase superfamily. LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A to form alpha-leucine ester residues.
Purification Method
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Physical Appearance
Sterile Filtered colorless solution.
Formulation
The LCMT1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1mM DTT and 0.15M NaCl.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Leucine carboxyl methyltransferase 1, LCMT1, LCMT, PPMT1, CGI-68.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSH MATRQR ESSITSCCST SSCDADDEGV RGTCEDASLC KRFAVSIGYW HDPYIQHFVR LSKERKAPEI NRGYFARVHG VSQLIKAFLR KTECHCQIVN LGAGMDTTFW RLKDEDLLPS KYFEVDFPMI VTRKLHSIKC KPPLSSPILE LHSEDTLQMD GHILDSKRYA VIGADLRDLS ELEEKLKKCN MNTQLPTLLI AECVLVYMTP EQSANLLKWA ANSFERAMFI NYEQVNMGDR FGQIMIENLR RRQCDLAGVE TCKSLESQKE RLLSNGWETA SAVDMMELYN RLPRAEVSRI ESLEFLDEME LLEQLMRHYC LCWATKGGNE LGLKEITY.
Background
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