Name
UBE2R2 Human
Cat. No.
MAG-4193
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
UBE2R2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 261 amino acids (1-238 a.a) and having a molecular mass of 29.6kDa (Molecular size on SDS-PAGE will appear higher). UBE2R2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
Ubiquitin-conjugating
enzyme E2 R2 (UBE2R2) is a member of the ubiquitin-conjugating
enzyme family. Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr
protein kinase implicated in cell growth and transformation. This protein is a protein similar to the
E2 ubiquitin conjugating enzyme
UBC3/CDC34. Studies propose
that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme
functions by regulating beta - TrCP
substrate recognition and induces its
interaction with beta-TrCP, enhancing beta-catenin degradation . UBE2R2 receives ubiquitin from the E1
complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes
monoubiquitination and 'Lys-48'-linked polyubiquitination. UBE2R2 may be implicated
in degradation of katenin. Among the diseases associated with UBE2R2 are cblc,
and herpes simplex.
Purification Method
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Physical Appearance
Sterile Filtered clear solution.
Formulation
UBE2R2 protein solution (0.25mg/ml) containing 20mM
Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Biological Activity
/
Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
CDC34B, E2-CDC34B, UBC3B, Ubiquitin carrier protein R2, Ubiquitin-conjugating
enzyme E2-CDC34B, Ubiquitin-protein ligase R2, Ubiquitin-conjugating enzyme E2
R2, EC 6.3.2.19.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MAQQQMT SSQKALMLEL KSLQEEPVEG FRITLVDESD LYNWEVAIFG
PPNTLYEGGY FKAHIKFPID YPYSPPTFRF LTKMWHPNIY ENGDVCISIL HPPVDDPQSG ELPSERWNPT
QNVRTILLSV ISLLNEPNTF SPANVDASVM FRKWRDSKGK DKEYAEIIRK QVSATKAEAE KDGVKVPTTL AEYCIKTKVP SNDNSSDLLY
DDLYDDDIDD EDEEEEDADC YDDDDSGNEE S
Background
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