Name
HEXA Human, Sf9
Cat. No.
MAG-4250
Tag/Conjugates
His
Source
Sf9,
Baculovirus cells.
Shipped
Shipped with Ice Packs
Description
HEXA produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 513 amino acids (23-529a.a.) and having a molecular mass of 59.2kDa.
Introduction
HEXA is the alpha subunit of the lysosomal enzyme beta-hexosaminidase which, combined with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules having N-acetyl hexosamines terminus. The two subunits composing Beta-hexosaminidase, alpha and beta, belong to the glycosyl hydrolases family and are encoded by distinct genes. Alpha subunit gene mutations can cause Tay-Sachs disease (GM2-gangliosidosis type I).
Purification Method
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Physical Appearance
Sterile Filtered colorless solution.
Formulation
HEXA protein solution (0. 5mg/ml) contains phosphate
buffered saline (pH7.4).
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 90% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Hexosaminidase
A (Alpha Polypeptide), N-Acetyl-Beta-Glucosaminidase Subunit Alpha, Beta-N-Acetylhexosaminidase
Subunit Alpha, Hexosaminidase Subunit A, EC 3.2.1.52, TSD, Beta-Hexosaminidase
Subunit Alpha, GM2 Gangliosidosis, Tay Sachs Disease, EC 3.2.1, Beta-hexosaminidase
subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, Hexosaminidase
subunit A, N-acetyl-beta-glucosaminidase subunit alpha.
Amino acid sequence
LWPWPQNFQT
SDQRYVLYPN NFQFQYDVSS AAQPGCSVLD EAFQRYRDLL FGSGSWPRPY LTGKRHTLEK NVLVVSVVTP
GCNQLPTLES VENYTLTIND DQCLLLSETV WGALRGLETF SQLVWKSAEG TFFINKTEIE DFPRFPHRGL
LLDTSRHYLP LSSILDTLDV MAYNKLNVFH WHLVDDPSFP YESFTFPELM RKGSYNPVTH IYTAQDVKEV
IEYARLRGIR VLAEFDTPGH TLSWGPGIPG LLTPCYSGSE PSGTFGPVNP SLNNTYEFMS TFFLEVSSVF
PDFYLHLGGD EVDFTCWKSN PEIQDFMRKK GFGEDFKQLE SFYIQTLLDI VSSYGKGYVV WQEVFDNKVK
IQPDTIIQVW REDIPVNYMK ELELVTKAGF RALLSAPWYL NRISYGPDWK DFYIVEPLAF EGTPEQKALV
IGGEACMWGE YVDNTNLVPR LWPRAGAVAE RLWSNKLTSD LTFAYERLSH FRCELLRRGV QAQPLNVGFC
EQEFEQT HHH HHH .
Background
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