Name
POLR2J3 Human
Cat. No.
MAG-4266
Tag/Conjugates
His
Source
E.coli.
Shipped
Shipped with Ice Packs
Description
POLR2J3 Human Recombinant produced in E. coli is a single polypeptide chain containing 138 amino acids (1-115) and having a molecular mass of 15.5 kDa. POLR2J3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
DNA-directed RNA polymerase II subunit RPB11-b2 (POLR2J3) is a member of the eukaryotic RPB11 RNA polymerase subunit family. POLR2J (DNA-directed RNA polymerase II subunit J) exists in 3 variants POLR2J1 (RPB11-a), POLR2J2 (RPB11-b1), and POLR2J3 (RPB11-b2). POLR2J3 is a DNA-dependent RNA polymerase which catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Purification Method
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Physical Appearance
Sterile Filtered colorless solution.
Formulation
The POLR2J3 solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 40% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioway's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
Polymerase (RNA) II (DNA Directed) Polypeptide J3, DNA-Directed RNA Polymerase II Subunit 11, DNA-Directed RNA Polymerase II Subunit RPB11-B2, DNA-Directed RNA Polymerase II Subunit J3, RNA Polymerase II Subunit B11-B2, POLR2J2, RPB11b2, RPB11b1.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGS MNAPPAF ESFLLFEGEK ITINKDTKVP NACLFTINKE DHTLGNIIKS QLLKDPQVLF AGYKVPHPLE HKIIIRVQTT PDYSPQEAFT NAITDLISEL SLLEERFRTC LLPLRLLP
Background
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