Cat. No.
MAK-0038
Product Name
Rat Anti-Tetanus Toxoid IgG
Storage
4°C
Species
Rat
Instructions
The assay was designed for use with serum or plasma obtained from rats 14 days or more after immunization with tetanus toxoid, at which point the immune response originates predominantly from IgG.
Introduction
Evaluation of the levels of anti-tetanus toxoid IgG following immunization with tetanus toxoid provides a useful indicator of aspects of the immune response. The rat anti-tetanus toxoid IgG test kit developed by Life Diagnostics, Inc. facilitates rapid and quantitative measurement of rat anti-tetanus toxoid IgG levels in serum or plasma samples.
Principle Of The Assay
The rat anti-tetanus toxoid IgG test kit is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses tetanus toxoid for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-rat IgG antibodies for detection. Test serum or plasma samples are diluted and incubated in the microtiter wells for 1 hour. The microtiter wells are subsequently washed, and HRP conjugate is added and incubated for 45 minutes. Anti-tetanus toxoid IgG molecules are thus sandwiched between immobilized tetanus toxoid and the detection antibody conjugate. The wells are then washed to remove unbound HRP-labeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of anti-tetanus toxoid IgG is proportional to the optical density of the test sample.
