Cat. No.
MAK-0082
Product Name
Mouse IgG1
Storage
4°C
Species
Mouse
Instructions
The mouse IgG1 ELISA kit is designed for measurement of IgG1 in mouse serum or plasma
Introduction
The mouse IgG1 ELISA kit is designed for measurement of IgG1 in mouse serum or plasma. The assay uses goat anti-mouse IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-mouse IgG1 antibodies for detection. The capture antibodies were cross-absorbed on mouse IgM and IgA agarose columns, and the detection antibodies were cross adsorbed on mouse IgM, IgA, IgG2a, IgG2b and IgG3 agarose thereby ensuring specificity for IgG1. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared mouse IgG1 standards. The microtiter wells are subsequently washed. HRP conjugate is added and incubated for 30 minutes. IgG1 molecules are thus sandwiched between the immobilization and detection antibodies.
The wells are then washed to remove unbound HRP-labeled antibodies. TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgG1 is proportional to the optical density of the test sample and is derived from a standard curve.
