Cat. No.
MAK-0094
Product Name
Monkey IgA
Storage
4°C
Species
Monkey
Instructions
The monkey IgA ELISA kit is designed for measurement of IgA in old world monkey serum or plasma.
Introduction
The monkey IgA ELISA kit is designed for measurement of IgA in old world monkey serum or plasma. The assay uses goat antimonkey IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-monkey IgA antibodies for detection. Both capture and detection antibodies were cross absorbed on monkey IgG and IgM agarose columns, thereby ensuring specificity for IgA. Cross-reactivity with immunoglobulins from other species has not been investigated. IgA is present in monkey serum, milk, and mucosal secretions at concentrations up to 2 mg/ml.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside monkey IgA standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgA molecules are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies. TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgA is proportional to the optical density of the test sample and is derived from a standard curve.
