Cat. No.
MAK-0097
Product Name
Monkey IgG (For Export)*
Storage
4°C
Species
Monkey
Instructions
The monkey IgG ELISA kit is designed for the measurement of IgG in monkey serum or plasma.
Introduction
The monkey IgG ELISA kit is designed for the measurement of IgG in monkey serum or plasma. Studies at LDI have shown that the kit works for IgG from Rhesus, Cynomolgus, Squirrel Monkey and Baboon. It does not recognize African Green monkey IgG.
Principle Of The Assay
Test samples and standards (100 µl) are incubated in the antibodycoated microtiter wells together anti-IgG-HRP conjugate (100 µl) for 90 minutes. The microtiter wells are subsequently washed to remove unbound HRP conjugate and TMB Reagent is added and incubated for 30 minutes. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgG is proportional to the optical density of the test sample and is derived from a standard curve.
