Cat. No.
MAK-0114
Product Name
Pig IgA
Storage
2-8°C
Species
Pig
Instructions
The pig IgA ELISA kit is designed for measurement of IgA (immunoglobulin A) in pig serum or plasma.
Introduction
The pig IgA ELISA kit is designed for measurement of IgA (immunoglobulin A) in pig serum or plasma. The assay uses antipig IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-pig IgA for detection. Both capture and detection antibodies were crossabsorbed on pig IgM and IgG agarose, thereby ensuring specificity for IgA. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared pig IgA standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgA molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgA is proportional to the optical density of the test sample and is derived from a standard curve.
