Cat. No.
MAK-0117
Product Name
Rabbit IgG
Storage
2-8°C
Species
Rabbit
Instructions
The Rabbit IgG ELISA kit is intended for measurement of IgG in serum and plasma.
Introduction
The Rabbit IgG ELISA kit is intended for measurement of IgG in serum and plasma. Goat anti-rabbit IgG heavy chain antibodies are used for solid phase (microtiter wells) immobilization, and horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG heavy chain antibodies are used for detection.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared Rabbit IgG standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgG molecules are thus sandwiched between the immobilization and detection antibodies.
The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgG is proportional to the optical density of the test sample and is derived from a standard curve.
