Cat. No.
MAK-0125
Product Name
Goat IgA
Storage
4°C
Species
Goat
Instructions
This ELISA kit is designed for measurement of IgA in goat serum and plasma.
Introduction
This ELISA kit is designed for measurement of IgA in goat serum and plasma. The assay uses rabbit anti-goat IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rabbit anti-goat IgA for detection. Both capture and detection antibodies react specifically with IgA. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside goat IgA standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgA molecules are thus sandwiched between the immobilization and detection antibodies. After washing the wells to remove unbound HRP-conjugate, TMB reagent is added and incubated for 20 minutes. This results in the development of a blue color. Color development is stopped by the addition of Stop solution, changing the color to yellow. Optical density is measured at 450 nm. The concentration of IgA is proportional to the optical density of the test sample and is derived from a standard curve.
