Cat. No.
MABL-3804
Application
functional assay, inhibit, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
Clone 29
From
Recombinant Antibody
Specificity
This antibody binds the human growth/differentiation factor 15 (GDF-15) also known as macrophage inhibitory cytokine 1. GDF-15 is a divergent member of the transforming growth factor-β (TGF- β) superfamily. While physiological expression is barely detectable in most somatic tissues in humans, GDF- 15 is abundant in placenta. It regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Highly elevated GDF-15 levels are mostly linked to pathological conditions including inflammation, myocardial ischemia, and notably cancer. GDF-15 is widely explored as a biomarker for disease prognosis.
Alternative Names
MIC-1; GDF-15 MIC1; PDF; PLAB; PTGFB; NAG-1; NRG-1; NAG1; NRG1;Growth/differentiation factor 15; growth and differentiation factor 15; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; Placental TGF-beta; Placental bone morphogenetic protein; Prostate differentiation factor; Clone#29; #29
UniProt
Q99988
Immunogen
The original mouse antibody was generated by immunizing BALB/c mice with human GDF-15 / MIC-1 protein. The humanized version was generated by grafting the variable domain regions of the original mouse antibody onto human framework regions.
Application Notes
This antibody can detect human GDF-15 protein in an ELISA and western blot. This antibody significantly reduces the neovascularization of endothelial cells produced by the GDF-15 protein. It also inhibits the growth of skin cancer, colon cancer, breast cancer or prostate cancer, pancreatic cancer, liver cancer, gastric cancer, inhibits angiogenesis inside/outside these tissues and inhibits the proliferation of cancer cells. The angiogenesis inhibitory activity was first confirmed in vitro through the tube formation assay and aortic ring branching assay. The inhibitory activity of this antibody was also tested in an in vivo angiogenesis assay using chick chorioallantoic membrane (CAM). It was reported that this antibody can inhibit angiogenesis in vivo to an excellent degree. This antibody is recommended for diagnosis of disease where GDF-15 is overexpressed (WO2019004550).
Antibody First Published
Note on publication
Size
285 μg Purified antibody.
Concentration
186 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 485.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 205⁰C.
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