Cat. No.
MABL-3815
Application
agonist, internalizing, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
1C1
From
Recombinant Antibody
Specificity
1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, and EphA4, or any EphB receptor family members. It is reported to cross react with mouse EphA2.
Alternative Names
EphA2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
UniProt
P29317
Immunogen
1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.
Application Notes
This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.
Antibody First Published
Jackson et al. A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo. Cancer Res. 2008 Nov 15;68(22):9367-74. PMID:19010911
Note on publication
Describes the generation of several human antibodies against the human ephrin type A receptor 2 and subsequent crystallography.
Size
296 μg Purified antibody.
Concentration
197 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 496.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 216⁰C.
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